Fig. 5

Amplicon sequencing reveals efficient editing by A3A and A3B base editors, but reduced precision with A3A. a Amplicon sequencing data for EMX1 and VEGFA editing in HEK293 cells 48 h post-transfection, expressed as percentage cumulative editing of the indicated locus. Data represent the mean ± SD from two independent experiments and include SNPs found at ≥ 1% of total reads per sample. UTC, untransfected control. All read counts can be found in Additional file 1: Table S4 and Table S5. b Amplicon sequencing data for EMX1 and VEGFA editing in HEK293 cells 48 h post-transfection, expressed as percentage nucleotide substitution at that position in the protospacer. The optimal base editing activity window is highlighted in grey. Data are from two independent experiments (circles, exp.1; triangles, exp.2) and include SNPs found at ≥ 1% of total reads per sample. For VEGFA, a connecting line joins the mean % editing. Untransfected cells served as a control. All read counts can be found in Additional file 1: Table S4 and Table S5. Raw data can be found in Additional file 2