Fig. 6
From: A role for actomyosin contractility in Notch signaling
Protrusion contacts and Myosin II localization. (a–a″) Filamentous actin in the basal protrusions co-localizes with myosin II heavy chain (visualized with Venus) in notum explant. Images are a z-projection ~ 2 μm basal to the cytoplasm of notum epithelium, where basal protrusions dominate. (b–b″) Phosphorylated MRLC (p-MRLC, spaghetti squash) localizes to the cell-proximal base of basal protrusions in fixed nota. (c–c″) Image from a fixed nota of the indicated genotype, GRASP (reconstituted membrane bound GFP) indicates the extent of cell-cell contacts between signal sending (pictured) and receiving cells. (c) A single SOP cell expressing GFP11 with neighboring epithelial cells expressing GFP1–10. (c′) A single SOP cell expressing GFP11 with neighboring epithelial cells not expressing any GFP1–10. (c″) Expression of both GFP fragments in all notum cell types. Scale bars: for b″, c = 5 μm; for a″, c′c″ =10 μm. See also Additional file 6: Figure S4