Fig. 6

Gonadal tissues from targeted knockout of the BCAR1 gene in channel catfish. a Histological examination of gonads 90 days post fertilization from 15 microinjected fish. Arrows indicate the position of the gonad. The agarose gel analysis indicates the genotypic sex of each fish. Males are shown as two bands, and females are shown as one band. Two fish (#9, #15) with male genotypes but female phenotypes are labeled in red. b Sequencing analysis of two KO clones. Target sequence is shown in red, mutations and indels are highlighted in green, and short black lines denote deletions WT, wild type