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Fig. 6 | BMC Biology

Fig. 6

From: Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis

Fig. 6

High-temperature regime in maize T0 transgenic plants enables high-frequency mutagenesis by LbCas12a. a Diagram of maize transformation and heat treatment. Transgenic plants are crossed with a wild type inbred B104 pollen donor. B, biallelic; M, monoallelic; W, wild type. b Mutation rates and genotyping results of T1 generation from two maize mutant lines, A842B-2-2 and A842B-5-1

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