Fig. 1

Sub-single cell profiling of soma and dendrite RNA. a Isolated single neurons were dissected to separate the soma and neurites, which were collected into separate tubes for amplification and RNA sequencing. b Overlap of expressed genes (≥ 10 reads) between soma and dendrites from the same original cell. Each horizontal bar shows the results from a single neuron. The Venn diagram depicts the general relationship between the somatic and dendritic transcriptomes observed in the chart, where the dendritic transcriptomes were largely a subset of the somatic transcriptome of the same cell. c Marker gene expression for several brain cell types. Samples (columns) are indicated as either dendritic samples (pink) or soma samples (blue). Expression values were normalized by library size using DESeq2. Cardiomyocyte markers are included as a control cell type that is electrically active but unrelated to brain cells