Fig. 3

Progressive destruction of the C. elegans pharynx by C. nematophagum. L1 stage C. elegans myo2::rfp (pharyngeal muscle marker) expressing strain VS21 were exposed to C. nematophagum for 1 h (a and b), 3 h (c and d) and 6 h (e and f). L1 stage C. elegans were stained with eosin Y to highlight chitosan in the pharynx prior to exposure to C. nematophagum for 1 h (g and h), 3 h (i and j) and 6 h (k and l). Panels b, d and f Depict DIC images, and a, c and e are the corresponding fluorescent images revealing the progressive breakdown of myo-2 labelled pharynx. Panels h, j and l depict DIC images, and g, i and k are the corresponding fluorescent images revealing the progressive breakdown of eosin Y-labelled chitosan of the pharynx. Large white arrow, posterior pharyngeal bulb and small white arrow anterior pharyngeal bulb, red arrow highlights the buccal cavity. All images at × 630