Fig. 7

Fragment-specific recovery of appropriate anterior-posterior gene expression. a The expression of genes asymmetrically expressed in either anterior (ANT; solid lines, cluster III) or posterior (POST; dashed lines, cluster IV) sea star larval territories was examined at 3 h post-bisection (hpb), 3 days post-bisection (dpb), and 6 dpb. The log fold change values for each gene in regenerating anterior or posterior fragments compared with non-bisected sibling control larvae is reported for each fragment (ANT/CONT and POST/CONT, respectively) over the regenerating time course sampled. Black lines show the detected expression of Frizz5/8 and Frizz9/10. b Model for recover of genes asymmetrically expressed along the anterior-posterior axis, with Frizz9/10 (blue) and Frizz5/8 (maroon) provided as examples. c Whole-mount fluorescent in situ hybridization illustrating the re-activation of Frizz9/10 (magenta) in the posterior aspect of regenerating anterior fragments beginning at 5 dpb and preceding the concentration of proliferating EdU⁺ cells (green) near the wound site. d Re-activation of Frizz5/8 (magenta) in the anterior aspect of regenerating posterior fragments beginning at 2 dpb and preceding the concentration of proliferating EdU⁺ cells near the wound site