Fig. 4

Phenotyping of ten lncRNA deletion mutants. a Viable brood sizes are presented with their standard deviations (blue area) and the 95% confidence interval of the mean (red area). Samples were compared to wild type animals using a pairwise two-sample t test with a multiple test (Bonferroni) correction. Samples are ordered by increasing p value and those found to be significant at (p ≤ 0.05) are shown to the left of the blue line (n = 15 animals/mutant). b Growth curves were compared to wild type animals, and those found not to be significantly different are shown by their mean across strains (black line) with the standard error of the mean (grey area). Those found to be significantly different from the control are shown individually as means only. Inset shows example images of the wild type (top) and linc-239 mutant (bottom) at 45 h post hatching with the computer-generated outlines, and computed area (black line = 500 μm)