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Fig. 5 | BMC Biology

Fig. 5

From: Identification of functional long non-coding RNAs in C. elegans

Fig. 5

RNAi-mediated knock-down of lncRNAs. a Viable brood sizes are presented with their standard deviations (blue area) and the 95% confidence interval of the mean (red area). Samples were compared to “empty vector” control animals using a pairwise two-sample t test with a multiple test (Bonferroni) correction. Samples are ordered by increasing p value, and those found to be significant at (p ≤ 0.05) are shown to the left of the blue line (n = 18 animals/mutant). Empty vector (EV), GFP and linc-340 RNAi are negative controls. b Growth curves were compared to “empty vector” animals, and those found to be not significantly different are shown by their mean across strains (black line) with the standard error of the mean (grey area). Those found to be significantly different from the control are shown individually as means only. Inset shows example images of the “empty vector” (top) and linc-239 RNAi (bottom) at 45 h post hatching with the computer-generated outlines, and computed area (black line = 500 μm). c RT-PCR analysis of RNAi knock-down efficiency for linc-239 and linc-339. Actin is used as loading control

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