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Fig. 3 | BMC Biology

Fig. 3

From: Behavioral heterogeneity in quorum sensing can stabilize social cooperation in microbial populations

Fig. 3

On-plate competition assay. Equal amount of P. aeruginosa XEN41 were spotted (5 μl) in triplicate on petri dish in a straight line with a crucial distance of 5.0 mm and cultured for 4 days on LB plate and 3 days on M9-milk plate. a Morphology and b cell distribution of XEN41 colonies on LB plate. c CFU and expression of lasB gene in the inner and outer colonies on LB plate. d Morphology and e cell distribution of XEN41 colonies on M9-milk plate. f CFU and expression of lasB gene in the inner and outer colonies on M9-milk plate. A.U., absorbance unit. Data shown in panels (c) and (f) are the percentage CFU (black bars) and lasB expression (gray bars) compared with an outer colony and represented by the mean values ± SD of three independent experiments. Statistical significance by two-tailed unpaired t test is indicated: **P < 0.01

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