Fig. 1
From: GSK-3β protects fetal oocytes from premature death via modulating TAp63 expression in mice
The expression patterns of GSK-3β in fetal and neonatal mouse ovaries. a GSK-3β localization in mouse ovaries from 13.5 dpc to 1 dpp. Mouse ovaries from 13.5 dpc, 15.5 dpc, 17.5 dpc, and 1 dpp were immunostained for GSK-3β (green) and germ cell marker DDX4 (red). The nucleus was stained by Hoechst (blue). GSK-3β was mainly expressed in the cytoplasm of both somatic cells (arrowhead) and oocytes (arrow) from 13.5 to 17.5 dpc. In the 1 dpp mouse ovary, GSK-3β was expressed in the cytoplasm of oocytes and the surrounding pre-granulosa cells of the formed primordial follicle (dashed line). b Phosphorylated GSK-3β at Ser9 (p-GSK-3β) was identified in mouse ovaries from 13.5 dpc to 1 dpp. The sections were immunostained for p-GSK-3β (green) and DDX4 (red). The nucleus was stained by Hoechst (blue). p-GSK-3β was scarcely expressed in oocytes before 17.5 dpc; part of the oocytes was stained for p-GSK-3β on 17.5 dpc (arrowhead), and the majority of oocytes expressed p-GSK-3β on 1 dpp (arrowhead). Scale bars, 200 μm