Fig. 1

mini-INTACT method affinity purifies cell-type-specific nuclei for epigenetic analysis in Drosophila. a Schematic of mini-INTACT method to affinity purify dopaminergic neurons from heads of Drosophila male flies after 4 days of social isolation or social enrichment. b Expression of SUN-tagged GFP (3XUAS-unc84-2XGFP) in dopaminergic neurons driven by TH-GAL4 in an adult male brain. c To assess the purity of dopaminergic nuclei, nuclei were obtained from a mixture of heads derived from flies expressing 10XUAS-unc84-tdTomfl under the control of the pan-neuronal elav-GAL4 driver (red) and flies expressing 3XUAS-unc84-2XGFP under the control of the TH-GAL4 driver (green). d After capturing green nuclei using bead-bound anti-GFP antibodies, red nuclei were washed away. e Bead-bound green nuclei were almost devoid of contaminating red nuclei