Fig. 4

Activity-regulated genes (ARGs) are upregulated in dopaminergic neurons of GH males and correlate with transcriptional repression. a A zoomed in scatter plot of GH versus SH mRNA values. Ddc (Fig. 2) and four ARG-TFs defined as such in [61] and showing high fold change in our data are highlighted. b Box plots of mRNA log fold change z-scores (GH is positive; SH is negative) for groups of ARGs from two different studies. Genes with log fold change lower than 1.5 in Chen et al.’s study [61] are not over-represented in GH flies (Chen et al. low). However, the last two groups are significantly over-represented in GH flies. c Genes repressed (red) or activated (blue) by the ARG-TF Cbt (from Bartok et al., 2015) are shown on the same z-score scale as in (b). Genes repressed by Cbt have significantly lower mRNA and activating mark H3K27ac and significantly higher repressive mark H3K27me3. Genes activated by Cbt show the reverse pattern