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Fig. 3 | BMC Biology

Fig. 3

From: In vivo assembly and trafficking of olfactory Ionotropic Receptors

Fig. 3

Heterogeneous requirement for the IR8a CREL N-glycosylation site in the localisation of tuning IRs. a Immunofluorescence with antibodies against GFP (green) and IR75a (magenta) on antennal sections of animals expressing the indicated transgenes in Or22a neurons (representing the field-of-view indicated in the cartoon). The arrowheads (in this and other panels) indicate the cilia of Or22a neurons; in neurons expressing EGFP:IR8aN669Q + IR75a (second row), the receptors are not detected in this sensory compartment, remaining restricted to the inner segment. Receptor localisation was determined by overlaying the fluorescence signal onto a bright-field channel, as shown in the merged images. Note that not all soma have a corresponding ciliated ending in these images, because this is a thin (14 μm) tissue section that does not include the entirety of all neurons. Genotypes are of the form UAS-EGFP:Ir8ax/UAS-Ir75a;Or22a-Gal4/+. Scale bar (for panels a, b): 10 μm. For each genotype, the phenotype was assessed in multiple sections of antennae from at least 20 animals from two independent genetic crosses. b Immunofluorescence with antibodies against GFP (green) and IR75c (magenta) on antennal sections of animals expressing the indicated transgenes in Or22a neurons. Genotypes are of the form UAS-EGFP:Ir8ax/UAS-Ir75c;Or22a-Gal4/+. For each genotype, the phenotype was assessed in multiple sections of antennae from at least 20 animals from two independent genetic crosses. c Immunofluorescence with antibodies against GFP (green) and RFP (magenta) on antennal sections of animals expressing the indicated transgenes in Or22a neurons. Genotypes are of the form UAS-EGFP:Ir8ax/+;Or22a-Gal4/UAS-mCherry:Ir84a. Scale bar: 10 μm. For each genotype, the phenotype was assessed in multiple sections of antennae from at least 30 animals from three independent genetic crosses

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