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Fig. 4 | BMC Biology

Fig. 4

From: In vivo assembly and trafficking of olfactory Ionotropic Receptors

Fig. 4

The IR8a CREL and CREL N-glycosylation site are important for ER export. a Immunofluorescence with antibodies against GFP (green) and RFP/Tomato (magenta) on antennal sections of animals expressing the indicated transgenes in Or22a neurons. The images on the right are high-magnification, single optical slices taken within the region shown in the lower-magnification view on the left in this and subsequent panels. Genotypes are of the form UAS-EGFP:Ir8ax/UAS-Ir75a;Or22a-Gal4/UAS-tdTomato:Sec61β. Scale bars: 5 μm. For each genotype, the phenotype was assessed in multiple sections of antennae from at least 20 animals from two independent genetic crosses, in this and the following panels. b Immunofluorescence with antibodies against GFP (green) and RFP (magenta) on antennal sections of animals expressing the indicated transgenes in Or22a neurons. Genotypes are of the form UAS-EGFP:Ir8ax/UAS-Ir75a;Or22a-Gal4/UAS-γCOP:mRFP. Scale bars: 5 μm. c Immunofluorescence with antibodies against GFP (green) and B9d1 (magenta) on antennal sections of animals expressing the indicated transgenes in Or22a neurons. Genotypes are of the form UAS-EGFP:Ir8ax/UAS-Ir75a;Or22a-Gal4/+. Scale bars: 5 μm.

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