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Fig. 5 | BMC Biology

Fig. 5

From: In vivo assembly and trafficking of olfactory Ionotropic Receptors

Fig. 5

The IR8a CREL N-glycosylation site is not essential for odour-evoked IR signalling. a Representative traces of the responses of Or22a neurons—those exhibiting the larger of the two spike amplitudes within this sensillum (black arrowhead)—expressing the indicated combinations of IRs, exposed to a 1-s pulse (black bar) of phenylacetic acid (1% v/v). Genotypes are of the form UAS-EGFP:Ir8ax/UAS-Ir84a;Or22a-Gal4/+, except for the control (Or22a-Gal4/+). b Quantification of the odour-evoked responses of the genotypes shown in a. Mean solvent corrected responses ±SEM are shown (n (number of sensilla) are indicated beneath each bar; mixed genders). Bars labelled with different letters are statistically different from each other (p < 0.05; Student’s t test with Benjamini and Hochberg correction for multiple comparisons). c Representative traces of the responses of Or22a neurons expressing the indicated combinations of IRs, exposed to a 1-s pulse (black bar) of propionic acid (1% v/v). Genotypes are of the form UAS-EGFP:Ir8ax/+;Or22a-Gal4/UAS-Ir75c, except for the control (Or22a-Gal4/+). d Quantification of the odour-evoked responses of the genotypes shown in c, presented as in b

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