Table 1 Characterization of canine jejunal tissues and jejunal enteroids using IHC
From: Derivation of adult canine intestinal organoids for translational research in gastroenterology
Tissue/enteroid | Stain/marker | Result | Location |
|---|---|---|---|
Enteroid | Keratin/epithelium | Pos | Epithelium |
Jejunum | Keratin/epithelium | Pos | Epithelium |
Enteroid | PAS/goblet cell | Pos | Goblet cells |
Jejunum | PAS/goblet cell | Pos | Goblet cells |
Enteroid | Chromogranin A/enteroendocrine cell | Pos | Epithelium |
Jejunum | Chromogranin A/enteroendocrine cell | Pos | Epithelium |
Enteroid | Vimentin/mesenchymal cell | Neg | Â |
Jejunum | Vimentin/mesenchymal cell | Pos | Submucosa, lamina propria vessels, muscularis |
Enteroid | Actin/mesenchymal cell | Neg | Â |
Jejunum | Actin/mesenchymal cell | Pos | Muscularis, lamina propria vessels |
Enteroid | c-Kit/leukocyte | Neg | Â |
Jejunum | c-Kit/leukocyte | Pos | Resident leukocytes |
Enteroid | T cell/T lymphocyte | Neg | Â |
Jejunum | T cell/T lymphocyte | Pos | Intraepithelial and lamina propria lymphocytes |
Enteroid | Lysozyme/Paneth cell | Neg | Epithelium |
Jejunum | Lysozyme/Paneth cell | Neg | Epithelium |