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Table 2 RNA in situ hybridization (ISH) probe details

From: Derivation of adult canine intestinal organoids for translational research in gastroenterology

Gene RNAscope® Probe Catalog no. Accession no. Target region
Leucine-rich repeat-containing G protein-coupled Receptor 5 Cl-LGR5 405651 XM_846738.2 517–1506
Sry-related high-mobility group box 9 Cl-SOX9 502551 NM_001002978.1 2–1536
Ephrin receptor B2 Cl-EPHB2 515361 XM_005617823.2 180–1757
Intestinal-specific alkaline phosphatase Cl-ALPI 515391 XM_534605.6 783–1791
Frizzled class receptor 5 Cl-FZD5 515371 XM_003640189.3 301–1514
neurogenin 3 Cl-NEUROG3 515271 XM _546140.1 2–575
Canine interleukin 17 (IL-17) Cl-IL17A 502541 NM_001165878 2–1178
Canine beta defensin 103 (CBD 103) Cl-CBD103 515411 NM_001129980 2–389
Canine cathelicidin (CATH) Cl-CAMP 515401 NM_001003359.1 2–607
Doublecortin-like kinase 1 (Dclk1) Cl-DCLK1 515311 XM_014107394.1 401–1547
Prostaglandin-receptor (EP4R) Cl-PTER4 499011 NM_001003054.1 535–1706