Table 2 RNA in situ hybridization (ISH) probe details
From: Derivation of adult canine intestinal organoids for translational research in gastroenterology
Gene | RNAscope® Probe | Catalog no. | Accession no. | Target region |
|---|---|---|---|---|
Leucine-rich repeat-containing G protein-coupled Receptor 5 | Cl-LGR5 | 405651 | XM_846738.2 | 517–1506 |
Sry-related high-mobility group box 9 | Cl-SOX9 | 502551 | NM_001002978.1 | 2–1536 |
Ephrin receptor B2 | Cl-EPHB2 | 515361 | XM_005617823.2 | 180–1757 |
Intestinal-specific alkaline phosphatase | Cl-ALPI | 515391 | XM_534605.6 | 783–1791 |
Frizzled class receptor 5 | Cl-FZD5 | 515371 | XM_003640189.3 | 301–1514 |
neurogenin 3 | Cl-NEUROG3 | 515271 | XM _546140.1 | 2–575 |
Canine interleukin 17 (IL-17) | Cl-IL17A | 502541 | NM_001165878 | 2–1178 |
Canine beta defensin 103 (CBD 103) | Cl-CBD103 | 515411 | NM_001129980 | 2–389 |
Canine cathelicidin (CATH) | Cl-CAMP | 515401 | NM_001003359.1 | 2–607 |
Doublecortin-like kinase 1 (Dclk1) | Cl-DCLK1 | 515311 | XM_014107394.1 | 401–1547 |
Prostaglandin-receptor (EP4R) | Cl-PTER4 | 499011 | NM_001003054.1 | 535–1706 |