Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly

Table 1 Parameters used in the model

		Value	Source
Motor parameters
v₀	Motor velocity	−0.01 μm/s	Estimated from contour velocity
D_c	Motor velocity fluctuation	5 × 10⁻⁴μm²/s	[55]
f₀	Motor stall force	−1.5 pN	[56]
k _on c _nuc	Motor concentration parameter	0.5 s⁻¹	Estimated
k_off	Motor detachment rate	0.1 s⁻¹	Estimated from [19]
k	Motor stiffness	300 pN/μm	[57]
y₀	Motor rest length	53 nm	[58]
Microtubule parameters
n_MT	Number of MTs in a nucleus	10	Measured here
〈R_1,2〉	Expected MT length	0.8 μm	[29, 59]
D_1,2	MT diffusion constant	\( 0.003/{R}_{1,2}^3 \)	[29]
Other parameters
d_SPB	Distance between SPBs	0.5 − 2.5 μm	Measured here
R_C	Nuclear envelope radius	1.5 μm	[29, 60]

ISSN: 1741-7007