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Fig. 5 | BMC Biology

Fig. 5

From: The impact of skin care products on skin chemistry and microbiome dynamics

Fig. 5

Underarm steroids and their longitudinal abundance. a–d Steroid molecular families in the armpits and their relative abundance over a 9-week period. Molecular networking was applied to characterize chemistries from the skin of 11 healthy individuals. The full network is shown in Additional file 1: Figure S4A, and networking parameters can be found here http://gnps.ucsd.edu/ProteoSAFe/status.jsp?task=284fc383e4c44c4db48912f01905f9c5 for MS/MS datasets MSV000081582. Each node represents a consensus of a minimum of 3 identical MS/MS spectra. Yellow nodes represent MS/MS spectra detected in armpits samples. Hexagonal shape represents MS/MS spectra match between skin samples and chemical standards. Plots are representative of the relative abundance of each compound over time, calculated separately from LC-MS1 data collected from the armpits of each individual. Steroids detected in armpits are a, dehydroisoandrosterone sulfate (m/z 369.190, rt 247 s), b androsterone sulfate (m/z 371.189, rt 261 s), c 1-dehydroandrostenedione (m/z 285.185, rt 273 s), and d dehydroandrosterone (m/z 289.216, rt 303 s). Relative abundance over time of each steroid compound is represented. Error bars represent the standard error of the mean calculated at each timepoint from four armpit samples from the right and left side of each individual separately. See also Additional file 1: Figures S4-S8

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