Fig. 3

Examples of hits in the GFP-reporter screen. eGFP pictures are shown on the left, DIC pictures on the right. Inhibitor-induced signal accumulation is highlighted by white arrowheads pointing towards the sites of accumulation. Numbers in brackets denote the unique identifier of the identified hits listed in Additional file 3: Table S3. a Untreated cells show cytoplasmic localization of both GFP-tagged reporter proteins. b Examples of inhibitors (streptonigrin, idarubicin HCl, and (+)-usnic acid) inducing nuclear accumulation in both reporter strains (Rpl7a-GFP and Rps9a-GFP, substance names in grey letters). c Examples of inhibitors (carmofur, vulpinic acid, and mycophenolic acid) causing nuclear accumulation only of the 60S reporter Rpl7a-GFP (red letters). d Examples of inhibitors (valsartan, all-trans retinoic acid, and visnagin) causing nuclear accumulation only of the 40S reporter Rps9a-GFP (blue letters)