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Table 1 Inhibitors causing the strongest pre-rRNA processing phenotypes and the most affected intermediate (for a complete list of all changing pre-rRNAs, see Additional file 3: Table S3)

From: Inhibiting eukaryotic ribosome biogenesis

Strongest pre-rRNA phenotype (±>1.5x)  Microscopy screen
60S hit 40S + 60S hit 40S hit
 All precursors gone Mycophenolic acid (19) Acivicin (1), streptonigrin (2)  
 27SA2 ↓*   Tanshinone IIA (7) Flubendazole (45)
 27SA2 Carmofur (17)   Valsartan (33), levocetirizine (63), cinanserin (65), doxepin (68), cytarabine (71), trans-4-cotininecarboxylic acid (112), gitoxigenin (113)
 27S↓   Acivicin (1) Ipriflavone (40)
 27S ↑   (+)-Usnic acid (4), syringetine-3-glucoside (13)  
 7S↓    Tunicamycin B (96), catalpol (97)
 7S ↑   Idarubicin HCl (3), cefaclor (54), desoximetasone (55) Fluphenazine 2HCl (53)**, pergolide mesylate (84)
 20S ↓   Antibiotic A-23187 (calcimycin) (5), nonactin (9) All trans retinoic acid (34), megestrol acetate (49), rotenone (93)
 A2-A3 sp. ↓   Curcumin (6)  
 A2-A3 sp. ↑ Vulpinic acid (18)   Fluphenazine 2HCl (53)**, rimcazole (57), thapsigargin (94), troleandomycin (95), veratramine (98), (−)-nicotine (100), L-penicillamine (101), picrotoxinin (102),
 D-A2   Icariin (39)
Additional strong pre-rRNA changes (manually curated)
 35S ↑ Parecoxib sodium (24), zerumbone (32) Tanshinone IIA (7), morine (8), nonactin (9), senecionine (15), bleomycin sulfate (16) Visnagin (35), zileuton (52), hexamethylenebisacetamide (66), indatraline HCl (70), cytarabine (71), naltrindole (72), uradipil HCl (74), DuP 697 (81), vindesine sulfate (82), clobenpropit (83), pergolide mesylate (84), catalpol (97), veratramine (98), ivermectin (99), (−)-nicotine (100), tryptanthrin (103), celastrol (106), isorhoifolin (108), narigenin-7-O-glucoside (110), leucomisine (115), tetrahydropapaverine HCl (116), tetrahydrolipistatin (121), phlorizine (123), diosmin (124),
 Aberrant 23S Carmofur (17), vulpinic acid (18) Tanshinone IIA (7), berberine HCl (14) Yangonin (127)
  1. * ↑ denotes accumulation, ↓ denotes reduction of the respective precursor
  2. **Listed twice due to equally strong effects
  3. (Substance identifier no., compare Additional file 3: Table S3)