Fig. 2

Both 13C-MFA and COBRA rely on measurements of metabolite uptake and secretion, cell biomass composition and growth rate, and information on reaction reversibility based on thermodynamic considerations. 13C-MFA further requires isotope tracing measurements and absolute concentrations of intracellular metabolites in a case of non-stationary 13C-MFA; COBRA relies on a variety of ‘omics’ datasets (genomics, transcriptomics, proteomics, and metabolomics). Inset COBRA image taken from [28]