Fig. 4
From: mTORC1/AMPK responses define a core gene set for developmental cell fate switching
Comparative expression parameters for rapamycin-induced development. a Log-phase cells in full growth media were adhered to cell well plates; after 2 h, fresh media were replaced with DB, and samples were analyzed by immunoblotting. Immunoblots are representative of three independent experiments. b YakA−/YakA-GFPOE cells in full growth media were adhered to a 6-well plate and in full media (left panel) or GDT media (right panel). Left panel—after 2 h, full media were replaced with fresh full media with or without rapamycin or with DB. Right panel—after 2 h, GDT media were replaced with fresh GDT media with or without rapamycin or with DB. Cell lysates were prepared at times indicated. Phosphorylation status of YakA was monitored by immunoblotting with α-GFP and PY20 to GFP-YakA. Immunoblots are representative of three independent experiments. c Log-phase cells in full growth media were adhered to cell well plates, in full media or GDT media. After 2h, full media were replaced with fresh media, and GDT media replaced with either fresh GDT media or with DB, all with or without rapamycin as indicated. Samples were taken for immunoblotting for protein gene expression as indicated. Immunoblots shown are representative of three independent experiments