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Fig. 5 | BMC Biology

Fig. 5

From: mTORC1/AMPK responses define a core gene set for developmental cell fate switching

Fig. 5

Comparative global gene expression changes by starvation and rapamycin-treated GDT media. Log-phase cells were adhered to cell well plates in GDT media. After 2 h, GDT media were replaced with DB or fresh GDT media with or without rapamycin. Cell samples were taken from controls and from the DB and rapamycin cells at varying times for RNA-seq. RNA-seq was in 3 replicates and up- and down-expressions determined as all significant differences (q < 0.1) comparing control values to developed values for DB or rapamycin cells after 30 min, 2 h, or 5 h. a Venn diagrams of induced/suppressed genes from starved or rapamycin-treated cells at times indicated, with total and overlapping gene numbers indicated and displayed proportionally. b Venn diagrams of induced/suppressed genes between 0.5 h of starvation and 0.5 or 2 h of rapamycin treatment, respectively, with total and overlapping gene numbers indicated, and displayed proportionally. Genes that overlap between 2 h of rapamycin treatment and 0.5 h of starvation are predicted to define classes of early regulated genes that are essential for growth-to-development transition (GDT), whereas gene classes regulated by starvation that do not overlap with rapamycin-treated cells are predicted to define non-essential classes (see Fig. 6)

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