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Fig. 6 | BMC Biology

Fig. 6

From: Analysis of the genetically tractable crustacean Parhyale hawaiensis reveals the organisation of a sensory system for low-resolution vision

Fig. 6

Photoreceptor projections visualised using opsin transgene reporters. a Confocal imaging of a live transgenic embryo carrying the PhOpsin1:EGFP-CAAX and PhOpsin2:mKate-CAAX reporters. Photoreceptor axons labelled by EGFP-CAAX (green) or mKate-CAAX (magenta) form the optic nerve (ONv) and terminate in the same neuropil. b, c Confocal imaging of the first neuropil of adult Parhyale carrying the PhOpsin1:EGFP-CAAX and PhOpsin2:mKate-CAAX reporters and stained with DAPI (in cyan). Photoreceptor projections labelled by EGFP-CAAX (green) terminate in the outer layer of the cup-shaped first neuropil, surrounding photoreceptor projections labelled by mKate-CAAX (magenta) which terminate at the interior. d 3D rendering of a confocal scan through the first optic neuropil of the brain of an adult Parhyale carrying both opsin reporters. Photoreceptor axons labelled by EGFP-CAAX and mKate-CAAX project to the first optic neuropil and do not extend to the second neuropil (located on the right). The optic nerve is located at the left of the image. e Lower magnification view of PhOpsin2:mKate-CAAX labelled photoreceptors, including the photoreceptor cell bodies at the retina (Re), the optic nerve (ONv) and the first optic neuropil (on the right of the image). Photoreceptor axons appear to cross each other before reaching the first neuropil. Scale bars, 10 μm

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