Fig. 3

Distribution of H3K27ac and H3K27me3 marks over the HoxD cluster and its flanking TADs in both control (Wt) and del(attP-Rel5)d9lac proximal and distal limb bud cells. a, b H3K27ac ChIP profiles from proximal (a) and distal (b) limb cells. Control is on top, and the del(attP-Rel5)d9lac profile is shown below along with the difference of deleted versus control ChIP data (del versus Wt). The arrowhead in a depicts the shared Hog and Tog start site (see also the divergent arrows below). The arrow in b indicates the CS93 enhancer. c, d H3K27me3 ChIP profiles from proximal (c) and distal (d) limb bud cells. Control is on top, and the del(attP-Rel5)d9lac track is shown below along with a comparison profile showing the difference between mutant and wild-type profiles. The data were averaged between different experiments (n is shown on the right). The red asterisks indicate artifactual peaks. The signal from the Hoxd9 region in the deleted allele corresponds to the Hoxd9/lacZ transgene