Fig. 6

Hoxd gene expression in the limbs in the absence of the 3′-TAD. a Normalized RNA-seq profiles of control (Wt) and inv(attP-Itga6)-mutant proximal (a) or distal (b) limb bud cells. Black arrows indicate the decreased RNA quantity over Hoxd12 and Hoxd11 in distal tissue (bottom two tracks) while expression has almost fully disappeared in proximal limb cells (top two tracks). b WISH of Hoxd4, Hoxd8, Hoxd9, Hoxd11, Hoxd13, and Evx2 in E12.5 forelimb buds. The arrowheads indicate digits II and V. c On the left, comparison of H3K27me3 signal over the HoxD cluster in either proximal (top two tracks) or distal (bottom two tracks) between control (Wt) and mutant inv(attP-Itga6) specimen. The CTCF sites are shown below. The arrows point to the extension of the H3K27me3 negative domain over the Hoxd11 region in mutant inv(attP-Itga6) distal cells (fourth track), when compared to control cells (third track). On the right, 4C-seq tracks showing interactions inside the HoxD cluster when Hoxd4 is used as bait (Wt: data from [34]). The arrows indicate a robust gain of interaction over Hoxd11 to Hoxd12 region in inv(attP-Itga6)-mutant distal cells