Skip to main content
Fig. 3 | BMC Biology

Fig. 3

From: FoxH1 represses miR-430 during early embryonic development of zebrafish via non-canonical regulation

Fig. 3

Mutated FoxH1 proteins for SID or FHD reveal dominant-negative effects on pri-miR-430 at 5hpf. a The FHD fused to VP16, EN, or GFP leads to enhanced upregulation in wild-type and MZsur mutants. Note highest upregulation for FHD-GFP constructs. b Sequences 5′ and 3′ to the FHD do not influence expression levels. A sur-mutated form of FHD fused to GFP does also not interfere with the expression level. c Addition of mutant proteins without DNA binding ability enhances the upregulation in wild type and MZsur mutant embryos. d Injection of SID-EN in both wild type and MZoep mutant embryos resulted in the downregulation of pri-miR-430 expression. The full-length protein consists of 472 aa. FHD (orange) is wild type or mutated to sur allele (*). SID (purple) can be wild type or replaced by VP16 (gray), GFP (green), or EN (brown). Olive-colored box following the FHD represents the EH1 domain which was included in earlier VP16, GFP, and EN constructs [10]. Error bars indicate standard error (SEM). All experiments were performed as biological triplicates of 5hpf embryos injected with the indicated mRNA at 1–2-cell stage. Relative normalized expression, standard error, and significance were calculated with the Bio-Rad CFX Manager 3.1 software (n. s. p ≥ 0.05; ***p < 0.001). For individual values, see also Additional file 5: Individual qPCR values

Back to article page