Fig. 4

A minimum of 20x depth of coverage on the MinION sequencer is sufficient to generate comparable gene sequence data to the Illumina MiSeq platform. a At 20x coverage on the MinION sequencer, comparisons with data generated on the Illumina MiSeq platform showed 98.74% sequence identity. b No notable selective bias occurred during library preparation and sequencing of individual genes using either the MiSeq or MinION platforms. Box plots show the percentage coverage for each of the 242 Pst genes sequenced for the four Pst isolates tested on the MinION and MiSeq platforms. c The number of SNPs per gene detected in each of the four MinION datasets was comparable to that from the MiSeq platform. Heatmaps represent the number of SNPs identified per gene (y-axis) for the four Pst isolates sequenced on the MinION and MiSeq platforms. Full details regarding the number of SNPs identified per gene are provided in Additional file 1: Table S9. In box plots a and b, bars represent median value, boxes signify the upper (Q3) and lower (Q1) quartiles, data falling outside the Q1–Q3 range are plotted as outliers