Fig. 9.

Regeneration occurs after removal of the main regions of active cell proliferation. A–C’ DIC images of an uncut cydippid (A) and cydippids after tentacle bulbs amputation (B–C’). Black dotted line rectangle in C shows the area corresponding to higher magnification on the right. D Schematic of the EdU incorporation experiment in cydippids in which both tentacle bulbs were amputated. E–G” Confocal stack projections of cydippids in which the tentacle bulbs were amputated oriented in a lateral view at different time points post amputation. The labeling corresponding to each panel is listed at the top of the columns, and the time following amputation is listed to the left of the rows. Dotted line rectangles in E, F, and G show the area corresponding to higher magnifications on the right. Dotted lines in E’ and E” and F’ and F” delimit the area corresponding to the endodermal canal. Nuclei of S-phase cells are labeled with EdU (magenta), and all nuclei are counterstained with DAPI (blue). Scale bars = 100 μm. Abbreviations: ts tentacle sheath, t tentacle, ec endodermal canal, tb tentacle bulb, hpa hours post amputation