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Fig. 10. | BMC Biology

Fig. 10.

From: Transcriptomic and functional analysis of the oosome, a unique form of germ plasm in the wasp Nasonia vitripennis

Fig. 10.

eRNAi phenotypes in Nv-coronin dsRNA-injected embryos. A1A3’ Pre-blastoderm stage. B1B3’ Mid-blastoderm stage. C1C3’ Mid-blastoderm stage. D1D3’ Mid-blastoderm stage. In each quadrant, the topmost image (A1, B1, C1, D1) shows a single-channel image of fluorescent in situ hybridization signal using a probe against Nv-bark beetle. The next image down contains single-channel images of DAPI staining (A2, B2, C2, D2). The merges of two single channels are in the bottom image of each quadrant (A3, B3, C3, D3). Enlarged views of the posterior pole (marked by white boxes in the whole embryo panels) appear to the right of each image (A1’D3’). The yellow arrowheads indicate that the germ plasm associated with a nucleus. The green arrow heads indicate the free germ plasm in the embryos. The white arrowheads indicate areas of injection damage in the embryos. All embryos are positioned with posterior side to the right and dorsal side on the top. Scale bar indicates 50 μm

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