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Fig. 6 | BMC Biology

Fig. 6

From: Transcriptomic and functional analysis of the oosome, a unique form of germ plasm in the wasp Nasonia vitripennis

Fig. 6

Development of embryos injected with eGFP dsRNA and probed with Nv-bark. A1–A3’ Pre-blastoderm stage. B1–B3’ Early blastoderm stage. C1C3’ Mid-blastoderm stage. D1–D3’ Mid-blastoderm stage. In each quadrant, the topmost image (A1, B1, C1, D1) shows a single channel image of fluorescent in situ hybridization signal using a probe against Nv-bark beetle. The next image down contains single-channel images of DAPI staining (A2, B2, C2, D2). The merges of two single channels are in the bottom image of each quadrant (A3, B3, C3, D3). Enlarged views of the posterior pole (marked by white boxes) appear to the right of each image (A1’D3’). The white dashed lines in B2 and B3 mark the edges of the embryos. The white arrowheads indicate areas of damage in the embryos. Some staining produced appreciable bleed-through from the fluorescent in situ detection into the DAPI channel, and instances of this are marked with blue arrow heads. All embryos are positioned with posterior side to the right and dorsal side on the top. Scale bar indicates 50 μm

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