Table 2 Detected phosphopeptides at 30 °C and 18 °C reactions, with each phosphorylated residue marked with star
Sequence | Theo.MH+ [Da] | DeltaM [ppm] | Site1 | Site2 | Site3 |
|---|---|---|---|---|---|
30 °C | |||||
 FS*EHYHDAWASR | 1585.62198 | − 0.15 | 2863 |  |  |
 IENS*WVYGENWSDSQK | 2021.82768 | 6.01 | 2878 |  |  |
 KIENS*WVYGENWSDSQK | 2149.92264 | 0.14 | 2878 |  |  |
 KIENS*WVYGENWSDS*QKAHPR | 2611.17254 | − 1.34 | 2888 |  |  |
 YKEPVRES*LK | 1328.66099 | 0.88 | 2916 |  |  |
 EPVRES*LK | 1037.5027 | 0.5 | 2916 |  |  |
 ALLAIGWSVEHS*EVDIPSTNR | 2374.14387 | 2.47 | 2930 |  |  |
 ALLAIGWSVEHSEVDIPS*TNRSSMR | 2835.34952 | 5.99 | 2936 |  |  |
 RQS*KSGGRPVDVVTDSATPFNYNPHPVDMTNLTLSR | 4052.92842 | 1.35 | 2946 |  |  |
 S*GGRPVDVVTDSATPFNYNPHPVDMTNLTLSR | 3553.64174 | 10.58 | 2948 |  |  |
 SGGRPVDVVTDS*ATPFNYNPHPVDMT*NLT*LSR | 3713.5744 | 12.04 | 2959 | 2973 | 2976 |
 SGGRPVDVVTDSATPFNYNPHPVDMT*NLTLSR | 3537.64682 | 9.43 | 2973 |  |  |
 S*QEFLK | 831.36481 | − 0.65 | 3033 |  |  |
 DRERS*QEFLK | 1387.63657 | − 0.1 | 3033 |  |  |
 ERS*QEFLK | 1116.50852 | 0.42 | 3033 |  |  |
18 °C | |||||
 KIENS*WVYGENWSDSQK | 2149.92264 | − 0.2 | 2878 |  |  |
 ERS*QEFLK | 1116.50852 | 1.07 | 2916 |  |  |
 ALLAIGWSVEHSEVDIPS*TNRSSMRR | 3007.44554 | 1.03 | 2936 |  |  |
 YKEPVRES*LK | 1328.66099 | − 0.45 | 3033 |  |  |