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Table 1 The cell lineage of the differentiated cell types at 30 hpf

From: From spiral cleavage to bilateral symmetry: the developmental cell lineage of the annelid brain

No. Cell type Lineage Support Last observed division rID
1 Posterior prototroch 1 1d-221 Refs. + (2/2) ~ 7 hpf r1365
2 Posterior prototroch 2 1d-222 Refs. + (2/2) ~ 7 hpf r1366
3 Posterior prototroch 3 1d-212 Refs. + (2/2) ~ 6.5 hpf r1363
4 Posterior prototroch 4 1a-221 Refs. + (2/2) ~ 7 hpf r215
5 Posterior prototroch 5 1a-222 Refs. + (2/2) ~ 7 hpf r216
6 Posterior prototroch 6 1a-212 Refs. + (2/2) ~ 7 hpf r213
7 Posterior prototroch 7 1b-221 Refs. + (2/2) ~ 7 hpf r361
8 Posterior prototroch 8 1b-222 Refs. + (2/2) ~ 7 hpf r362
9 Posterior prototroch 9 1b-212 Refs. + (2/2) ~ 7 hpf r359
10 Posterior prototroch 10 1c-221 Refs. + (2/2) ~ 7 hpf r899
11 Posterior prototroch 11 1c-222 Refs. + (2/2) ~ 7 hpf r900
12 Posterior prototroch 12 1c-212 Refs. + (2/2) ~ 7 hpf r897
13 Anterior prototroch 1 1d-1221 (3/3) ~ 8.5 hpf r1356
14 Anterior prototroch 2 1d-211 Refs. + (2/2) ~ 6.5 hpf r1362
15 Anterior prototroch 3 1a-1212 (3/3) ~ 8.5 hpf r208
16 Anterior prototroch 4 1a-122 (3/3) ~ 7 hpf r209
17 Anterior prototroch 5 1a-211 Refs. + (2/2) ~ 7 hpf r212
18 Anterior prototroch 6 1b-1212 (3/3) ~ 8.5 hpf r354
19 Anterior prototroch 7 1b-122 (3/3) ~ 7 hpf r355
20 Anterior prototroch 8 1b-211 Refs. + (2/2) ~ 7 hpf r358
21 Anterior prototroch 9 1c-1212 (3/3) ~ 8.5 hpf r892
22 Anterior prototroch 10 1c-122 (3/3) ~ 7 hpf r893
23 Anterior prototroch 11–12 1c-211 Refs. + (2/2) ~ 7 hpf r896
24 Left head kidney 1d-11,221 (5/5) ~ 7 hpf r1133
25 Right head kidney 1c-11221 (5/5) ~ 7 hpf r613
26 Dorsally migrating 1st wave 1d-1222 (5/5) ~ 8.5 hpf r1357
27 Dorsally migrating 2nd wave 1d-121 (5/5) ~ 7 hpf r1352
28 Gland most right (GRR) 1b-112211121 (3/4) ~ 15.5 hpf r267
29 Gland middle right (GMR) 1b-11221222 (3/3) ~ 13.5 hpf r295
30 Gland middle (GMM) 1b-121122 (3/3) ~ 13 hpf r353
31 Gland middle left (GML) 1a-11221222 (3/3) ~ 13.5 hpf r126
32 Gland most left (GLL) 1a-112211121 (3/3) ~ 15.5 hpf r118
33 Apoptotic upper right 1c-12111 (3/3) ~ 10.5 hpf r838
34 Apoptotic lower right 1b-1122112 (3/3) ~ 11 hpf r290
35 Apoptotic upper left 1a-12111 (3/3) ~ 10.5 hpf r158
36 Apoptotic lower left 1a-1122112 (3/3) ~ 11 hpf r122
37 Ampullary cell right 1c-1112 (3/3) ~ 9 hpf r367
38 Ampullary cell left 1c-1111 (3/3) ~ 9 hpf r366
39 Large dorsal apical cell 1d-1112 (5/5) ~ 10 hpf r905
40 Crescent cell 1c-112121222 (4/4) ~ 17.5 hpf r574
41 First axon apical organ 1d-1111 (3/3) ~ 10 hpf r904
42 Larval eye pigment cell right 1c-121121b (3/3) ~ 16 hpf r842
43 Larval eye photoreceptor right, chat+ 1c-121121a (3/3) ~ 16 hpf r841
44 Larval eye pigment cell left 1a-1211211 (3/3) ~ 16 hpf r206
45 Larval eye photoreceptor left, chat+ 1a-1211212 (3/3) ~ 16 hpf r207
46 chat+ apical dorsal, phc2+, first bilateral axons 1a-11111 (3/3) ~ 11.5 hpf r5
47 chat+ apical ventral peripheral (AVP) 1a-11212112b (3/3) ~ 16.5 hpf r86
48 chat+ apical ventral medial (AVM) 1a-11211212b (3/3) ~ 18 hpf r50
49 chat+ right lateral bigger (RLP) 1c-112112222ba (4/5) ~ 20 hpf r525
50 chat+ right lateral ventral (RLV) 1b-11211221 (3/4) ~ 16.5 hpf r241
51 chat+ left lateral bigger (LLP) 1d-112112222aa (3/3) ~ 20 hpf r1113
52 chat+ left lateral ventral (LLV) 1d-112112221nn (2/2) ~ 28/~ 29 hpf r1125/r1121
53 The right apical cell with axon, 5HT+, phc2+ 1b-1121111 (1/1) ~ 14.8 hpf r226
54 The most dorsal serotonergic, 5HT+ 1c-112122122a (1/1) ~ 17.5 hpf r583
55 Asymmetricserotonergic, 5HT+ 1c-112222211bb (1/1) ~ 22 hpf r834
56 phc2+ close to the right apical cell with axon, more dorsal 1b-1121112 (1/1) ~ 14.8 hpf r227
57 phc2+ 1a-1122121 (1/1) ~ 11.5 hpf r124
58 phc2+ 1a-11112b (1/1) ~ 15.6 hpf r24
59 phc2+, close to the right apical cell with axon, more ventral 1b-1122121 (1/1) ~ 11.5 hpf r292
60 phc2+ close to the crescent cell, more ventral 1d-112121 (1/1) ~ 10 hpf r1128
61 phc2+ close to the crescent cell, more dorsal 1d-1121221 (1/1) ~ 15.2 hpf r1130
62 Dorsal medial phc2+ 1d-1121222 (1/1) ~ 15.2 hpf r1131
  1. The numbers in the first column correspond to the numbering in Fig. 3F. The column “Lineage” contains the consensus lineage name based on the literature and multiple time-lapse recordings. The “Support” column indicates the number of time-lapse recordings with the given cell lineage/the number of total time-lapse recordings analyzed for a given cell type. “Refs” indicates additional support from published literature [23]. The column “Last observed division” refers to the time point at which the last cell division was observed. Since most of the cells in this table show terminal differentiation characteristics, we consider the time of the last observed division being identical to cell cycle exit. The “rID” column contains the reference ID of the given cell type which corresponds to the rID in the tracking files (Additional files 4 and 7 and the tracking files of individual embryos provided in online data repository [28]) and the consensus lineage tree (Additional file 10: Figure S2)