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Table 1 Number of reads and barcodes generated via MinION sequencing

From: Rapid, large-scale species discovery in hyperdiverse taxa using 1D MinION sequencing

  Set 1: two flowcells Set 2: one flowcell Combined (sets 1 and 2)*
# Specimens 4275 4519 8699
Resequencing (re-pooled) 2172 2211
# reads/# reads > 600 bp 7,035,075/3,703,712 7,179,121/2,652,657 NA
Initial sequencing (all) 3,069,048/1,942,212 4,853,363/2,250,591
Resequencing (re-pooled) 3,966,027/1,761,500 2,325,758/402,066
# demultiplexed reads 898,979 (24.3%) 647,152 (24.4%)
Initial sequencing (all) 562,434 (29%) 561,383 (24.9%)
Resequencing (re-pooled) 336,545 (19%) 85,769 (21.3%)
Combined results of original and resequencing runs
 # specimens with ≥ 5× coverage 4227 (98.9%) 4287 (94.9%) 8428 (96.9%)
 # MAFFT barcodes < 1% Ns 3797 (88.8%) 3476 (76.9%) 7221 (83%)
 # MAFFT+AA barcodes 3771 (88.2%) 3459 (76.5%) 7178 (82.5%)
 # RACON barcodes 3797 (88.8%) 3476 (76.9%) 7221 (83%)
 # RACON+AA barcodes 3788 (88.6%) 3461 (76.6%) 7194 (82.7%)
 # Consolidated barcodes 3762 (88%) 3446 (76.3%) 7155 (82.3%)
 # Consolidated barcodes (non-phorids removed) 3727 (87.2%) 3426 (75.8%) 7059 (81.1%)
 # mOTUS (2/3/4%)    705/663/613
  1. *One plate was accidentally sequenced in both runs, duplicates removed for combined set