From: Rapid, large-scale species discovery in hyperdiverse taxa using 1D MinION sequencing
Set 1: two flowcells | Set 2: one flowcell | Combined (sets 1 and 2)* | |
---|---|---|---|
# Specimens | 4275 | 4519 | 8699 |
Resequencing (re-pooled) | 2172 | 2211 | |
# reads/# reads > 600 bp | 7,035,075/3,703,712 | 7,179,121/2,652,657 | NA |
Initial sequencing (all) | 3,069,048/1,942,212 | 4,853,363/2,250,591 | |
Resequencing (re-pooled) | 3,966,027/1,761,500 | 2,325,758/402,066 | |
# demultiplexed reads | 898,979 (24.3%) | 647,152 (24.4%) | |
Initial sequencing (all) | 562,434 (29%) | 561,383 (24.9%) | |
Resequencing (re-pooled) | 336,545 (19%) | 85,769 (21.3%) | |
Combined results of original and resequencing runs | |||
# specimens with ≥ 5× coverage | 4227 (98.9%) | 4287 (94.9%) | 8428 (96.9%) |
# MAFFT barcodes < 1% Ns | 3797 (88.8%) | 3476 (76.9%) | 7221 (83%) |
# MAFFT+AA barcodes | 3771 (88.2%) | 3459 (76.5%) | 7178 (82.5%) |
# RACON barcodes | 3797 (88.8%) | 3476 (76.9%) | 7221 (83%) |
# RACON+AA barcodes | 3788 (88.6%) | 3461 (76.6%) | 7194 (82.7%) |
# Consolidated barcodes | 3762 (88%) | 3446 (76.3%) | 7155 (82.3%) |
# Consolidated barcodes (non-phorids removed) | 3727 (87.2%) | 3426 (75.8%) | 7059 (81.1%) |
# mOTUS (2/3/4%) | 705/663/613 |