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Table 1 Number of reads and barcodes generated via MinION sequencing

From: Rapid, large-scale species discovery in hyperdiverse taxa using 1D MinION sequencing

 

Set 1: two flowcells

Set 2: one flowcell

Combined (sets 1 and 2)*

# Specimens

4275

4519

8699

Resequencing (re-pooled)

2172

2211

# reads/# reads > 600 bp

7,035,075/3,703,712

7,179,121/2,652,657

NA

Initial sequencing (all)

3,069,048/1,942,212

4,853,363/2,250,591

Resequencing (re-pooled)

3,966,027/1,761,500

2,325,758/402,066

# demultiplexed reads

898,979 (24.3%)

647,152 (24.4%)

Initial sequencing (all)

562,434 (29%)

561,383 (24.9%)

Resequencing (re-pooled)

336,545 (19%)

85,769 (21.3%)

Combined results of original and resequencing runs

 # specimens with ≥ 5× coverage

4227 (98.9%)

4287 (94.9%)

8428 (96.9%)

 # MAFFT barcodes < 1% Ns

3797 (88.8%)

3476 (76.9%)

7221 (83%)

 # MAFFT+AA barcodes

3771 (88.2%)

3459 (76.5%)

7178 (82.5%)

 # RACON barcodes

3797 (88.8%)

3476 (76.9%)

7221 (83%)

 # RACON+AA barcodes

3788 (88.6%)

3461 (76.6%)

7194 (82.7%)

 # Consolidated barcodes

3762 (88%)

3446 (76.3%)

7155 (82.3%)

 # Consolidated barcodes (non-phorids removed)

3727 (87.2%)

3426 (75.8%)

7059 (81.1%)

 # mOTUS (2/3/4%)

  

705/663/613

  1. *One plate was accidentally sequenced in both runs, duplicates removed for combined set