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Table 1 Number of reads and barcodes generated via MinION sequencing

From: Rapid, large-scale species discovery in hyperdiverse taxa using 1D MinION sequencing

 Set 1: two flowcellsSet 2: one flowcellCombined (sets 1 and 2)*
# Specimens427545198699
Resequencing (re-pooled)21722211
# reads/# reads > 600 bp7,035,075/3,703,7127,179,121/2,652,657NA
Initial sequencing (all)3,069,048/1,942,2124,853,363/2,250,591
Resequencing (re-pooled)3,966,027/1,761,5002,325,758/402,066
# demultiplexed reads898,979 (24.3%)647,152 (24.4%)
Initial sequencing (all)562,434 (29%)561,383 (24.9%)
Resequencing (re-pooled)336,545 (19%)85,769 (21.3%)
Combined results of original and resequencing runs
 # specimens with ≥ 5× coverage4227 (98.9%)4287 (94.9%)8428 (96.9%)
 # MAFFT barcodes < 1% Ns3797 (88.8%)3476 (76.9%)7221 (83%)
 # MAFFT+AA barcodes3771 (88.2%)3459 (76.5%)7178 (82.5%)
 # RACON barcodes3797 (88.8%)3476 (76.9%)7221 (83%)
 # RACON+AA barcodes3788 (88.6%)3461 (76.6%)7194 (82.7%)
 # Consolidated barcodes3762 (88%)3446 (76.3%)7155 (82.3%)
 # Consolidated barcodes (non-phorids removed)3727 (87.2%)3426 (75.8%)7059 (81.1%)
 # mOTUS (2/3/4%)  705/663/613
  1. *One plate was accidentally sequenced in both runs, duplicates removed for combined set