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Table 2 Accuracy of MinION as assessed by Illumina barcodes. The MinION barcodes were trimmed to the 313 bp that were sequenced using Illumina. The overall optimal strategy is “Consolidated (namino = 2)”. Optimal congruence values are highlighted in bold

From: Rapid, large-scale species discovery in hyperdiverse taxa using 1D MinION sequencing

Dataset # compared with Illumina/ # 3% mOTUs Accuracy
Mean (stdev)
%Ns # barcodes with errors/# > 3% errors mOTU richness deviation between MinION and Illumina barcodes
  2% 3% 4%
MAFFT 6291/641 99.6136 (0.37) 0.18 (0.2) 4473/30 -3 (−0.44%) − 2 (−0.31%) −11 (−1.85%)
RACON 6291/645 99.5097 (0.48) < 0.001 (0.01) 4494/36 12 (1.72%) 2 (0.31%) −3 (−0.5%)
MAFFT+AA (namino = 1) 6269/635 99.9689 (0.19) 1.19 (0.84) 273/27 −6 (−0.89%) −6 (− 0.94%) − 6 (−1.02%)
MAFFT+AA (namino = 2) 6269/635 99.9802 (0.15) 1.28 (0.92) 216/28 −8 (−1.19%) −6 (− 0.94%) − 14 (− 2.37%)
MAFFT+AA (namino = 3) 6269/633 99.9685 (0.18) 1.25 (0.91) 310/27 −8 (−1.19%) − 8 (− 1.26%) −17 (−2.9%)
RACON+AA (namino = 1) 6273/639 99.9636 (0.19) 0.48 (0.47) 392/26 − 4 (−0.59%) − 4 (− 0.63%) − 13 (− 2.19%)
RACON+AA (namino = 2) 6273/635 99.9736 (0.16) 0.55 (0.55) 288/26 −5 (− 0.74%) −8 (− 1.26%) −14 (− 2.36%)
RACON+AA (namino = 3) 6273/636 99.9684 (0.17) 0.57 (0.6) 345/28 −1 (− 0.15%) −7 (− 1.1%) −13 (− 2.19%)
Consolidated (namino = 1) 6229/639 99.9849 (0.12) 0.41 (0.44) 191/26 −2 (− 0.29%) − 2 (− 0.31%) −3 (− 0.5%)
Consolidated (namino = 2) 6251/638 99.9859 (0.11) 0.46 (0.5) 184/25 − 2 (− 0.29%) − 3 (− 0.47%) −5 (− 0.83%)
Consolidated (namino = 3) 6245/639 99.9795 (0.12) 0.44 (0.49) 285/26 −4 (− 0.59%) −2 (− 0.31%) −4 (− 0.67%)