Table 2 Accuracy of MinION as assessed by Illumina barcodes. The MinION barcodes were trimmed to the 313 bp that were sequenced using Illumina. The overall optimal strategy is “Consolidated (namino = 2)”. Optimal congruence values are highlighted in bold
From: Rapid, large-scale species discovery in hyperdiverse taxa using 1D MinION sequencing
Dataset | # compared with Illumina/ # 3% mOTUs | Accuracy Mean (stdev) | %Ns | # barcodes with errors/# > 3% errors | mOTU richness deviation between MinION and Illumina barcodes | ||
|---|---|---|---|---|---|---|---|
2% | 3% | 4% | |||||
MAFFT | 6291/641 | 99.6136 (0.37) | 0.18 (0.2) | 4473/30 | -3 (−0.44%) | − 2 (−0.31%) | −11 (−1.85%) |
RACON | 6291/645 | 99.5097 (0.48) | < 0.001 (0.01) | 4494/36 | 12 (1.72%) | 2 (0.31%) | −3 (−0.5%) |
MAFFT+AA (namino = 1) | 6269/635 | 99.9689 (0.19) | 1.19 (0.84) | 273/27 | −6 (−0.89%) | −6 (− 0.94%) | − 6 (−1.02%) |
MAFFT+AA (namino = 2) | 6269/635 | 99.9802 (0.15) | 1.28 (0.92) | 216/28 | −8 (−1.19%) | −6 (− 0.94%) | − 14 (− 2.37%) |
MAFFT+AA (namino = 3) | 6269/633 | 99.9685 (0.18) | 1.25 (0.91) | 310/27 | −8 (−1.19%) | − 8 (− 1.26%) | −17 (−2.9%) |
RACON+AA (namino = 1) | 6273/639 | 99.9636 (0.19) | 0.48 (0.47) | 392/26 | − 4 (−0.59%) | − 4 (− 0.63%) | − 13 (− 2.19%) |
RACON+AA (namino = 2) | 6273/635 | 99.9736 (0.16) | 0.55 (0.55) | 288/26 | −5 (− 0.74%) | −8 (− 1.26%) | −14 (− 2.36%) |
RACON+AA (namino = 3) | 6273/636 | 99.9684 (0.17) | 0.57 (0.6) | 345/28 | −1 (− 0.15%) | −7 (− 1.1%) | −13 (− 2.19%) |
Consolidated (namino = 1) | 6229/639 | 99.9849 (0.12) | 0.41 (0.44) | 191/26 | −2 (− 0.29%) | − 2 (− 0.31%) | −3 (− 0.5%) |
Consolidated (namino = 2) | 6251/638 | 99.9859 (0.11) | 0.46 (0.5) | 184/25 | − 2 (− 0.29%) | − 3 (− 0.47%) | −5 (− 0.83%) |
Consolidated (namino = 3) | 6245/639 | 99.9795 (0.12) | 0.44 (0.49) | 285/26 | −4 (− 0.59%) | −2 (− 0.31%) | −4 (− 0.67%) |