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Table 2 Accuracy of MinION as assessed by Illumina barcodes. The MinION barcodes were trimmed to the 313 bp that were sequenced using Illumina. The overall optimal strategy is “Consolidated (namino = 2)”. Optimal congruence values are highlighted in bold

From: Rapid, large-scale species discovery in hyperdiverse taxa using 1D MinION sequencing

Dataset# compared with Illumina/ # 3% mOTUsAccuracy
Mean (stdev)
%Ns# barcodes with errors/# > 3% errorsmOTU richness deviation between MinION and Illumina barcodes
 2%3%4%
MAFFT6291/64199.6136 (0.37)0.18 (0.2)4473/30-3 (−0.44%)− 2 (−0.31%)−11 (−1.85%)
RACON6291/64599.5097 (0.48)< 0.001 (0.01)4494/3612 (1.72%)2 (0.31%)−3 (−0.5%)
MAFFT+AA (namino = 1)6269/63599.9689 (0.19)1.19 (0.84)273/27−6 (−0.89%)−6 (− 0.94%)− 6 (−1.02%)
MAFFT+AA (namino = 2)6269/63599.9802 (0.15)1.28 (0.92)216/28−8 (−1.19%)−6 (− 0.94%)− 14 (− 2.37%)
MAFFT+AA (namino = 3)6269/63399.9685 (0.18)1.25 (0.91)310/27−8 (−1.19%)− 8 (− 1.26%)−17 (−2.9%)
RACON+AA (namino = 1)6273/63999.9636 (0.19)0.48 (0.47)392/26− 4 (−0.59%)− 4 (− 0.63%)− 13 (− 2.19%)
RACON+AA (namino = 2)6273/63599.9736 (0.16)0.55 (0.55)288/26−5 (− 0.74%)−8 (− 1.26%)−14 (− 2.36%)
RACON+AA (namino = 3)6273/63699.9684 (0.17)0.57 (0.6)345/28−1 (− 0.15%)−7 (− 1.1%)−13 (− 2.19%)
Consolidated (namino = 1)6229/63999.9849 (0.12)0.41 (0.44)191/26−2 (− 0.29%)− 2 (− 0.31%)−3 (− 0.5%)
Consolidated (namino = 2)6251/63899.9859 (0.11)0.46 (0.5)184/25− 2 (− 0.29%)− 3 (− 0.47%)−5 (− 0.83%)
Consolidated (namino = 3)6245/63999.9795 (0.12)0.44 (0.49)285/26−4 (− 0.59%)−2 (− 0.31%)−4 (− 0.67%)