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Fig. 8 | BMC Biology

Fig. 8

From: DPF is a cell-density sensing factor, with cell-autonomous and non-autonomous functions during Dictyostelium growth and development

Fig. 8

Cells that overexpress DPF regulate prespore/spore patterning. a WT or C-GFPOE cells were identically plated on DB agar at a density of 400 × 103 cells/cm2 for development and followed over time. Shown are similar time frame images including both DIC and GFP fluorescence. b A 9:1 mixed population of WT or C-GFPOE cells were plated for development and followed over time. Shown are two time frame images including both DIC and GFP fluorescence (see Additional file 8: Movie S1). c A 99:1 mixed population of WT or C-GFPOE cells were plated for development and developed to the slug stage (left) or to terminal differentiation (right). Shown are confocal images including both DIC and GFP fluorescence, with prespore/prestalk and spore/stalk regions indicated

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