Fig. 8

Cells that overexpress DPF regulate prespore/spore patterning. a WT or C-GFP^OE cells were identically plated on DB agar at a density of 400 × 10³ cells/cm² for development and followed over time. Shown are similar time frame images including both DIC and GFP fluorescence. b A 9:1 mixed population of WT or C-GFP^OE cells were plated for development and followed over time. Shown are two time frame images including both DIC and GFP fluorescence (see Additional file 8: Movie S1). c A 99:1 mixed population of WT or C-GFP^OE cells were plated for development and developed to the slug stage (left) or to terminal differentiation (right). Shown are confocal images including both DIC and GFP fluorescence, with prespore/prestalk and spore/stalk regions indicated