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Fig. 9 | BMC Biology

Fig. 9

From: DPF is a cell-density sensing factor, with cell-autonomous and non-autonomous functions during Dictyostelium growth and development

Fig. 9

Cell-autonomous and non-autonomous functions of DPF in development. a Log phase growing DPF- cells were adhered in a 12-well plate under fresh, naïve DB starvation buffer at indicated cell densities for 24 h. b Log phase growing DPF- or DPF-OE Dictyostelium were plated under fresh, naïve DB starvation buffer at indicated cell densities for 24 h. c WT, DPF-, DPFOE, and DPF-OE cells were adhered in fresh, naïve DB media at a density of 100 × 103 or 400 × 103 cells/cm2 and developed for 5 h. Cell lysates were prepared and immunoblotted to α-CAR1 and α-actin. d WT and DPF- cells were adhered at a density of 100 × 103 cells/cm2 and developed for 5 h using fresh, naïve DB media, or > 30-kDa conditioned media from WT, DPFOE, or DPF- cells following starvation in DB for 5 h. Cell lysates were prepared and immunoblotted to α-CAR1 and α-actin

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