Fig. 9

Cell-autonomous and non-autonomous functions of DPF in development. a Log phase growing DPF^- cells were adhered in a 12-well plate under fresh, naïve DB starvation buffer at indicated cell densities for 24 h. b Log phase growing DPF^- or DPF^-OE Dictyostelium were plated under fresh, naïve DB starvation buffer at indicated cell densities for 24 h. c WT, DPF^-, DPF^OE, and DPF^-OE cells were adhered in fresh, naïve DB media at a density of 100 × 10³ or 400 × 10³ cells/cm² and developed for 5 h. Cell lysates were prepared and immunoblotted to α-CAR1 and α-actin. d WT and DPF^- cells were adhered at a density of 100 × 10³ cells/cm² and developed for 5 h using fresh, naïve DB media, or > 30-kDa conditioned media from WT, DPF^OE, or DPF^- cells following starvation in DB for 5 h. Cell lysates were prepared and immunoblotted to α-CAR1 and α-actin