Fig. 2

Co-stimulation at 594 nm and 488 nm attenuates the inactivation of eNpHR3.0-mediated currents during prolonged photo-stimulation in a mean power-dependent manner. a Sample voltage-clamp recording from a single cell illustrating eNpHR3.0-mediated currents in response to photo-stimulation at 594 nm (5 mW) alone (top) or in combination with 488 nm at variable power levels (middle and bottom). Power levels indicated refer to 488-nm light. b Dependence of inactivation on the power of 488-nm light. c The rescue effect of 488-nm light on the inactivation of eNpHR3.0-dependent currents depends on its mean, rather than peak, power. Top: continuous 488-nm stimulation (left) is equally effective in attenuating inactivation as compared to pulsed (1 kHz, 20/80% on/off) stimulation at constant mean power (right). Bottom: continuous 488-nm stimulation (left) is more effective in attenuating inactivation as compared to pulsed (1 kHz, 20/80% on/off) stimulation at constant peak power (right). d For quantification, I_late measured during pulsed stimulation was normalized to I_late obtained for the respective continuous-stimulation trials. Each symbol represents a single cell. Data are presented as mean ± SEM. **P < 0.01