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Fig. 7 | BMC Biology

Fig. 7

From: Optimized photo-stimulation of halorhodopsin for long-term neuronal inhibition

Fig. 7

Wavelength and duration dependence of chloride loading due to photo-stimulation of eNpHR3.0. a Sample gramicidin perforated-patch recording of membrane potential in response to puff application of isoguvacine (Iso, 100 μM, 2 s) before (Control) and after photo-stimulation at 488 nm for 30 s (5 mW). Dotted lines indicate resting (Vrest) and peak (Vpeak) membrane potential measured before photo-stimulation. Note that Vpeak approximates EGABA under our recording conditions. b Quantification of Vrest and Vpeak before and after photo-stimulation. c, d As in a and b, but photo-stimulation was performed for 120 s at 488 nm (5 mW). e, f As in a and b, but photo-stimulation was performed for 120 s at 594 nm (5 mW). Experiments were performed at P4–10. Data are presented as mean ± SEM. n.s. not significant, **P < 0.01, ***P < 0.001

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