Fig. 2

Ribosome profiling under multiple temperatures. a Schematic of the ribosome profiling experiment. BY4741 cells harboring the F-Luc^UUG reporter plasmid were grown at multiple temperatures, and then transcriptome-wide translation was analyzed by ribosome profiling. b Wiggle track image of F-Luc^UUG reporter mRNA at multiple temperatures. Ribosome-protected fragments (RPFs) on the F-Luc^UUG reporter mRNA in cells cultured at 20, 30, or 37 °C, in units of rpm (reads per million mapped reads from two replicates at each temperature). The RPF tracks were normalized to the mRNA levels (see the “Materials and methods” section) at each temperature to reflect the changes in translation efficiencies (ΔTE). c Translation efficiency (TE) values, calculated as ribosome density (RPF reads on F-Luc mRNA normalized to total number of RPF reads mapped) divided by the mRNA density (mRNA reads of reporter mRNA normalized to total number of mRNA reads mapped) (ribo-density/mRNA-density), for the F-Luc^UUG reporter mRNA from both biological replicates at 20 °C (blue), 30 °C (black), and 37 °C (red). Each point represents the TE value for the F-Luc reporter from one replicate, and the horizontal solid line represents the mean