Fig. 7
From: Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae
Identification and analysis of NCC uORFs with the greatest changes in TE at 20 °C and 37 °C. a Heatmaps of ΔTEuORF values for all translated NCC uORFs (N = 1223), ranked according to ΔTE. The scale on the top indicates the range of ΔTE values. The dotted boxes indicate the 100 uORFs with the greatest decreases in TEuORF (TE_down) or the greatest increases in TEuORF (TE_up) at either 20 °C or 37 °C with respect to 30 °C. b Boxplot analysis of the 100 uORFs with the greatest decrease (TE_down) or increase (TE_up) in TEuORF at 20 °C, identified as described in a. The median ΔTEuORF values for each group are shown below the plot (mΔTE). All indicates all translated NCC uORFs (N = 1223). c Same as in b but for ΔTEuORF at 37 °C. d Boxplot analysis of the distance between the start site of the uORF and the 5′-end of the mRNA. The dotted horizontal line indicates the median distance of All translated NCC uORF start codons from the 5′-ends of the mRNAs. e Boxplot analysis of the distance between uORF start sites and the downstream mORF start (mAUG) codons. The dotted horizontal line indicates the median distance of All translated NCC uORF start codons from the mORF start codons