Fig. 8
From: Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae
Correlations between changes in uORF and mORF translation suggest possible novel cases of uORF-mediated translational regulation. a–f Correlation analysis between ΔTEuORF and ΔTEmORF at multiple growth temperatures. Each circle in the plots represents a uORF. The circles are color-coded according to the relative ribosome occupancy of the uORF (RROuORF; color key is shown at the bottom) which is the ribosome occupancy on the uORF (ROuORF) normalized to the ribosome occupancy on the mORF (ROmORF). ROuORF is the RPF density on the uORF at 30 °C normalized to its length, and ROmORF is the RPF density on the mORF at 30 °C normalized to its length. The plot is divided into four quadrants (Q1–Q4). The number of uORFs in each quadrant is shown on every plot. Multiple sets of uORFs are used in this analysis. a, b The uORFs with ≥ 2-fold changes in TE (activation or repression) at FDR < 0.1 were analyzed (highlighted circles in red and blue in Additional file 1: Figures S4A and S4B). a Analysis of changes observed at 20 °C. b Analysis of changes observed at 37 °C. c, d NCC uORFs with the greatest changes in TE (TE_down and TE_up) at 20 °C (c) and 37 °C (d) as described in Fig. 7a were analyzed. e, f All translated AUG uORFs (N = 136) were analyzed for changes in translation at 20 °C (e) and 37 °C (f). For a–f, cases representing the uORFs with a possible canonical inhibitory function for the uORFs are indicated with an arrow