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Fig. 2 | BMC Biology

Fig. 2

From: Peripheral cathepsin L inhibition induces fat loss in C. elegans and mice through promoting central serotonin synthesis

Fig. 2

Functional inactivation of CPL-1 suppressed fat accumulation in C. elegans. a Schematic representation of C. elegans CPL-1 and the immunoblot for CPL-1 protein in three cpl-1 mutants ok360, qx304, and yq89. SP, signal peptide; PP, propeptide; MP, mature peptide. b The representative images and quantification of Oil Red O staining in N2 and cpl-1-mutant worms induced by supplementation of glucose or palmitic acid. c Real-time PCR analysis of the expression of cpl-1 in control or cpl-1 knockdown worms, n = 3 independent growths. act-1 was used as the reference gene in real-time PCR analysis. d The representative images and quantification of Oil Red O staining in control or cpl-1 knockdown worms induced by supplementation of glucose or palmitic acid. For Oil Red O staining quantifications, the data were obtained from 3 independent experiments and 3000 stained worms were used in each experiment. The data in bd are presented as mean ± SEM; for b, two-way ANOVA; and for c, d, two-tailed Student’s t test was used for statistical analysis. *p < 0.05; **p < 0.01; ***p < 0.001

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