Fig. 5

Knockdown of cpl-1 induced body fat loss via a central serotonin signaling. a Representative images of Oil Red O staining in N2, mod-1(ok103), ser-6(tm2146), and mod-1(ok103);ser-6(tm2146) worms fed with control or cpl-1 RNAi bacteria. b Quantification of Oil Red O staining in N2, mod-1(ok103), ser-6(tm2146), and mod-1(ok103);ser-6(tm2146) worms fed with control or cpl-1 RNAi bacteria; the data were presented as the proportion of fat loss, n = 3 independent growths. c–e Real-time PCR analysis of genes involved in lipolysis, fatty acid β-oxidation, and lipogenesis in N2 (c), tph-1(mg280) (d), and mod-1(ok103);ser-6(tm2146) (e) worms fed with control or cpl-1 RNAi bacteria. act-1 was used as the reference gene in real-time PCR analysis, n = 3 independent growths. The data in b–e are presented as mean ± SEM. For b, one-way ANOVA; for c–e, two-tailed Student’s t test was used for statistical analysis. *p < 0.05; **p < 0.01; ***p < 0.001. n.s., not significant