Table 1 S. cerevisiae strains used in this study
Strain (lab ID no.) | Genotype | Reference |
|---|---|---|
RL285-16C (SHY WT) (4928) | MATa his3Δ1 ura3Δ0 | [6] |
mpc1Δ (SHY9) (4929) | MATa his3Δ1 ura3Δ0 mpc1::kanMX | [6] |
mpc2Δmpc3Δ (SHY14) (4932) | MATa his3Δ1 ura3Δ0 mpc2::natMX mpc3::hphMX | [6] |
mpc1Δmpc2Δmpc3Δ (SHY15) (4933) | MATa his3Δ1 ura3Δ0 mpc1::kanMX mpc2::natMX mpc3::hphMX | [6] |
YPH499 (WT) (1501) | MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 | [63] |
tom20Δ (1273) | MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tom20::URA3 pYEP-TOM22 | [62] |
tom70Δ (1183) | ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tom70::HIS3 | |
tim18Δ (1383) | MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim18::kanMX6 | [48] |
tim22-14 (1370) (YPH499 22-M4) | MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim22-M4 (amino acid alterations in Tim22-14: I11M, K16R, E21K, G31R, N37D, F63L, A85T, T86A, K120R, C141S, Y153H, M193 T, K194Q) | [48], this study |
tim12-21 (2462) (YPH-BG-12-1) | MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim12::ADE2 pFL39-TIM12-1ts (amino acid alterations in Tim12-21: S7G, V14D, A22E, D64A) | [55] |
tim17-4 (1758) (YPH-BG17-9d) | MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 BG17-9d (tim17-4) (amino acid alteration in Tim17-4: C10R) | |
tim17-5 (1759) (YPH-BG17-21-7) | MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 BG17-21-7 (tim17-5) (amino acid alterations in Tim17-5: P42L, R109G, S115P) | |
WT for Tim10 mutants (5118) | MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim10::ADE2 pFL39-TIM10 | [26] |
Tim10-L26Q (5210) | MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim10::ADE2 pFL39-TIM10-L26Q | [26] |
tim8Δtim13Δ (5084) | MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim8::natNT2 tim13::hphNT1 tim10::ADE2 pFL39-TIM10 | [26] |
Tim10-L26Q tim8Δtim13Δ (5206) | MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim8::natNT2 tim13::hphNT1 tim10::ADE2 pFL39-TIM10-L26Q | [26] |
BY4741 (WT) (1354) | MATa ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 | Euroscarf |
mpc1Δ (4774) | MATa ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 mpc1::kanMX4 | Euroscarf |
mpc2Δ (4775) | MATa ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 mpc2::kanMX4 | Euroscarf |
mpc3Δ (4776) | MATa ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 mpc3::kanMX4 | Euroscarf |