Skip to main content

Table 1 S. cerevisiae strains used in this study

From: The mitochondrial carrier pathway transports non-canonical substrates with an odd number of transmembrane segments

Strain (lab ID no.)GenotypeReference
RL285-16C (SHY WT) (4928)MATa his3Δ1 ura3Δ0[6]
mpc1Δ (SHY9) (4929)MATa his3Δ1 ura3Δ0 mpc1::kanMX[6]
mpc2Δmpc3Δ (SHY14) (4932)MATa his3Δ1 ura3Δ0 mpc2::natMX mpc3::hphMX[6]
mpc1Δmpc2Δmpc3Δ (SHY15) (4933)MATa his3Δ1 ura3Δ0 mpc1::kanMX mpc2::natMX mpc3::hphMX[6]
YPH499 (WT) (1501)MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1[63]
tom20Δ (1273)MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tom20::URA3 pYEP-TOM22[62]
tom70Δ (1183)ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tom70::HIS3[60, 61]
tim18Δ (1383)MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim18::kanMX6[48]
tim22-14 (1370) (YPH499 22-M4)MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim22-M4 (amino acid alterations in Tim22-14: I11M, K16R, E21K, G31R, N37D, F63L, A85T, T86A, K120R, C141S, Y153H, M193 T, K194Q)[48], this study
tim12-21 (2462)
(YPH-BG-12-1)
MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim12::ADE2 pFL39-TIM12-1ts (amino acid alterations in Tim12-21: S7G, V14D, A22E, D64A)[55]
tim17-4 (1758) (YPH-BG17-9d)MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 BG17-9d (tim17-4) (amino acid alteration in Tim17-4: C10R)[46, 47], this study
tim17-5 (1759) (YPH-BG17-21-7)MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 BG17-21-7 (tim17-5) (amino acid alterations in Tim17-5: P42L, R109G, S115P)[46, 47], this study
WT for Tim10 mutants (5118)MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim10::ADE2 pFL39-TIM10[26]
Tim10-L26Q (5210)MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim10::ADE2 pFL39-TIM10-L26Q[26]
tim8Δtim13Δ (5084)MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim8::natNT2 tim13::hphNT1 tim10::ADE2 pFL39-TIM10[26]
Tim10-L26Q tim8Δtim13Δ (5206)MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 tim8::natNT2 tim13::hphNT1 tim10::ADE2 pFL39-TIM10-L26Q[26]
BY4741 (WT) (1354)MATa ura3Δ0 his3Δ1 leu2Δ0 met15Δ0Euroscarf
mpc1Δ (4774)MATa ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 mpc1::kanMX4Euroscarf
mpc2Δ (4775)MATa ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 mpc2::kanMX4Euroscarf
mpc3Δ (4776)MATa ura3Δ0 his3Δ1 leu2Δ0 met15Δ0 mpc3::kanMX4Euroscarf
\