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Fig. 2. | BMC Biology

Fig. 2.

From: Spatiotemporal mapping of RNA editing in the developing mouse brain using in situ sequencing reveals regional and cell-type-specific regulation

Fig. 2.

Relative expression of Adar1, Adar2, and Adar3 transcripts. a The spatial distribution of Adar1 (red), Adar2 (green), Adar2 inactive (yellow), Adar2 active (purple), and Adar3 (cyan) transcripts and the regional selection outlined for each developmental stage. The scale bar is 1 mm. b Total number of reads for Adar1, Adar2, and Adar3 for each developmental stage (Additional files 789, and 10), each replicate is represented by a dot. As different developmental stages have different levels of background autofluorescence, resulting in varying quality thresholds for optimal read quality, the read counts in adults are lower than expected. An increase in expression of Adar2 was observed from E15 to adult by non-parametric Kruskal-Wallis (p = 8.7E−4). c The relative proportion of reads, an average based on the replicates from each developmental stage, for Adar1, Adar2, and Adar3 showing a developmental increase in the proportion of the inactive Adar2 splice isoform. The proportions of active and inactive Adar2 transcripts are based on the derived level of auto-editing of Adar2. d Regional read proportions of Adar1, Adar2, and Adar3, presented as in c. Hippocampus and neocortex could not be outlined for E15 and hence only data for thalamus and hippocampus are presented for this stage. Asterisks indicate the level of significance for the observed differences, *** p < 0.001

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