Fig. 1

Peripheral blood CD4⁺ T cells are responsible for the anti-CTLA-4 antibody effect. Flow cytometric analysis of gut-homing T cells in the blood 10 days after isotype antibody (Ctrl) or anti-CTLA-4 mAb treatment. a Representative flow plots depicting activated gut-homing T cells (β7⁺CD44⁺) (left) and quantification of activated gut-homing T cells (right). The upper panel was gated from live CD4⁺ subset and the lower gated from live CD8⁺ subset. b Representative flow plots depicting activated gut-homing CD4⁺ T cells (β7⁺CD62L⁻) (left) and quantification of activated gut-homing T cells (right). The numbers indicate the percentage of total cells in the indicated gate. The right bar graph shows a summary of the data for 5 mice in each group (mean ± SEM). For all graphs: **P < 0.01 and ***P < 0.001