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Fig. 6 | BMC Biology

Fig. 6

From: Identification of berberine as a novel drug for the treatment of multiple myeloma via targeting UHRF1

Fig. 6

The in vitro effects of UHRF1 on the proliferation of MM cells. a RPMI-8266 and MM.1S cells were transfected with either NC or UHRF1 siRNA #2100 nM for 48 h, and cell viability was determined using MTT assay. The data were presented as the mean ± SD obtained from three independent experiments. Significance was determined by Student’s t test, **p < 0.01 versus NC-siRNA transfection groups. b, c Targeting of UHRF1 with UHRF1-siRNA #2 transfection inhibited the colony formation ability of RPMI-8266 cells. Histogram and statistics indicating the relative number of colonies per 1000 plated cells. The data were presented as the mean ± SD obtained from three independent experiments. Significance was determined by Student’s t test, **p < 0.01, ***p < 0.001 versus NC-siRNA transfection groups. d RPMI-8266 and MM.1S cells were transfected with NC-siRNA or UHRF1-siRNA #2100 nM for 24 h, cells were subsequently treated with DMSO or BBR (25, 50 μM) for 24 h, and cell viability was determined using MTT assay. Percent cell viability was normalized (as 100%) for NC- or UHRF1-siRNA #2 controls, respectively. The data were presented as the mean ± SD obtained from three independent experiments. Significance was determined by Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001 versus NC-siRNA transfection groups. e Overexpression of UHRF1 promoted the cell proliferation of RPMI-8266 and MM.1S. RPMI-8266 and MM.1S cells were transfected with Lentiviral Flag-UHRF1 or vector carrying a puromycin selection marker. After puromycin selection for 2 weeks, cell viability was determined at 12 h, 24 h, 48 h, and 72 h using MTT assay. The data were presented as the mean ± SD obtained from three independent experiments. Significance was determined by Student’s t test, *p < 0.05, ***p < 0.001 versus vector. f Overexpression of UHRF1 renders RPMI-8266 and MM.1S cells more resistant to BBR. RPMI-8266 and MM.1S cells were transfected with Lentiviral Flag-UHRF1 or vector carrying a puromycin selection marker. After puromycin selection for 2 weeks, cell viability was determined at 48 h using MTT assay. The data were presented as the mean ± SD obtained from three independent experiments. Significance was determined by Student’s t test, *p < 0.05, **p < 0.01 versus vector

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